We narrowed to 15,989 results for: LEA

1. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

   Plasmid

   #80325

   Purpose

   Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

   Depositor

   Daniel Schoenberg

   Article

   Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

   Insert

   PXDNL-003 (PXDNL Human)
   

   Tags

   Biotin

   Expression

   Mammalian

   Mutation

   H170A and H256A

   Promoter

   CMV

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. NET39 Short pmRFP-N2 (451)

   Plasmid

   #61981

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET39 (PPAPDC3 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Short form of NET39

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pHL836

   Plasmid

   #62827

   Purpose

   Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   gfp
   ipex leader
   hfq leader
   

   Use

   Synthetic Biology

   Tags

   ompC leader and st7 RBS

   Expression

   Bacterial

   Promoter

   pLlacO-1 and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pHL1954

   Plasmid

   #62831

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   M8::ryhB leader
   hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::sodB leader and st7 RBS

   Expression

   Bacterial

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pHL1969

   Plasmid

   #62832

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   M8::ryhB leader
   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::sodB leader

   Expression

   Bacterial

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pHL2107

   Plasmid

   #62841

   Purpose

   Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   m8::micC leader
   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::ompC leader

   Expression

   Bacterial

   Promoter

   pConNoHind, pLlacO-1, and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-PKAsub(T/A)-YPet-NES

   Plasmid

   #138205

   Purpose

   Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub(T/A)-YPet-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNESx2-EGFP-C1-PABDx2-Spo20

   Plasmid

   #220082

   Purpose

   PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Tags

   X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

   Expression

   Mammalian

   Mutation

   amino acids 51-91, tandem dimer

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN-A112C-GpA

   Plasmid

   #191238

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn1-FLEX-CybSEP2-WPRE

    Plasmid

    #207661

    Purpose

    Monitoring neuropeptide release

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Cytochrome b-561 (Cyb561 Mouse)
    

    Use

    AAV

    Mutation

    Histidine 86 and 159 to alanines, inserted 2 X SE…

    Promoter

    hSynapsin

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pASK-mSAN1-Streptag

    Plasmid

    #117165

    Purpose

    Expresses Strep-tagged murine SAN1 nuclease in bacteria

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    mSAN1 (Fam120b Mouse)
    

    Tags

    Streptag

    Expression

    Bacterial

    Promoter

    tetR/tetA

    Available Since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LBR pEGFP-N2 (646)

    Plasmid

    #61996

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LBR (LBR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. phTERT-cp PS Intein tTA

    Plasmid

    #242032

    Purpose

    Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Cp PS Intein tTA
    

    Expression

    Mammalian

    Mutation

    The CMV promoter was replaced with the hTERT prom…

    Promoter

    hTERT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AP4mS_PPVs_P3_cLuc

    Plasmid

    #119300

    Purpose

    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

    Plasmid

    #86674

    Purpose

    Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS E225A/D227A
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NKP38 GFP (1009)

    Plasmid

    #62021

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    AYTL1 (LPCAT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only