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pSS02:cyt-Peredox-mCherry_DS

Plasmid

#161747

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry_DS

Expression

Plant

Mutation

amino acid substitution D115S in first T-Rex doma…

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin

Plasmid

#118710

Purpose

Lentiviral TET-ON inducible GFP-Progerin

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C EGFR EABR

Plasmid

#234994

Purpose

For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

EGFR-EABR (EGFR Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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AICSDP-1:PXN-EGFP

Plasmid

#87420

Purpose

Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

PXN Homology Arms with linker-EGFP (PXN Human)

Use

CRISPR; Donor template

Tags

linker-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCXN2-HA-AT1R-YFP

Plasmid

#101659

Purpose

Expresses AT1R with HA tag and YFP in mammalian cells.

Depositor

Yusuke Ohba

Article

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Insert

Angiotensin II type 1 receptor (AGTR1 Human)

Tags

HA and YFP (Venus)

Expression

Mammalian

Promoter

CAG promoter

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-FLAG-AKAP-Lbc

Plasmid

#67571

Purpose

Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

A-kinase anchoring protein Lbc (AKAP13 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

R2604G in AKAP

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only