Addgene

We narrowed to 788 results for: Nf1

Showing: 301 - 330 of 788 results

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MSCV-Arkadia

Plasmid

#180372

Purpose

expressing mouse Arkadia proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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SIK1_pCSdest

Plasmid

#53936

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SIK1 (SIK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-GFP-Flag-UTR

Plasmid

#183235

Purpose

To express FLAG tagged eGFP

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJ3M-Mst1 K59R

Plasmid

#12204

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst1 (STK4, MST1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive: K59R

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA2

Plasmid

#180362

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human RLIM N581K

Plasmid

#232242

Purpose

Mammalian expression of Human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

Human RLIM N581K (RLIM Human)

Use

Tags

Expression

Mammalian

Mutation

N581K

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 human RLIM N581K

Plasmid

#232241

Purpose

Protein expression of GST-human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

GST-human RLIM N581K (RLIM Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N581K

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA5

Plasmid

#180366

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA4

Plasmid

#180365

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA6

Plasmid

#180367

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA3

Plasmid

#180364

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2-HygR-EGFP

Plasmid

#167188

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-FUS-IDR

Plasmid

#183236

Purpose

Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Use

Tags

FLAG tag and Fusion to an IDR from the FUS protei…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K118-119R

Plasmid

#63564

Purpose

Expression of human H2A with K118R and K119R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 118 and Lysine 119 are now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-IKZF2-V2-V5

Plasmid

#107391

Purpose

Mammalian expression of IKZF2-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2-V2 (IKZF2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13-15R

Plasmid

#63563

Purpose

Expression of human H2A with K13R and K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 13 and Lysine 15 are now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pLV-rtTA-zeo

Plasmid

#183233

Purpose

Lentiviral vector expressing the rtTa gene

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

rtTA-T2A-Zeo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsTTP_1-313_AH

Plasmid

#148879

Purpose

Mammalian Expression of HsTTP_1-313

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsTTP_1-313 (ZFP36 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and EF1

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-RLIM-IRES-mCherry

Plasmid

#232243

Purpose

Mammalian expression of RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

RLIM-GFP-IRES-mCherry (RLIM Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1752

Plasmid

#143974

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF180 (ZNF180 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF1373

Plasmid

#143954

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF143 (ZNF143 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1374

Plasmid

#143955

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF143 (ZNF143 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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