We narrowed to 611 results for: prime editing
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Plasmid#181811Purposems2 incorporated pegRNA-1.1DepositorInsertMS2-pegRNA
UseCRISPRAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_60bp
Plasmid#174856PurposeExpress forward pegRNA mediating 991-bp deletion and 60-bp insertionDepositorInsertHEK3_pegF_60bp insertion
ExpressionMammalianPromoterU6Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegR_60bp
Plasmid#174857PurposeExpress reverse pegRNA mediating 991-bp deletion and 60-bp insertionDepositorInsertHEK3_pegR_60bp insertion
ExpressionMammalianPromoterU6Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACME
Plasmid#186717PurposeACME dual-deaminase CRISPR base editorDepositorInsertACME
UseCRISPRTagsNucleoplasmin NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2-3HA
Plasmid#169853PurposeMammalian Expression, SpyCas9 prime editor with 3HA-tagDepositorInsertSpCas9-H840A-M-MLV-3HA
Tags3XHA tag and bpSV40 NLSExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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lenti-EGFR-L858R-tmpknot-epegRNA
Plasmid#214092PurposeLentiviral vector expressing epegRNA to induce EGFR L858R mutationDepositorInsertEGFR L858R epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G12C-tmpknot-epegRNA
Plasmid#214094PurposeLentiviral vector expressing epegRNA to induce KRAS G12C mutationDepositorInsertKRAS G12C epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_GBA-4
Plasmid#199274PurposepegRNA used to correct GBA (c. 1226 A > G) mutationDepositorInsertGBA 1226GtoA pegRNA
ExpressionMammalianAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA-SV40-puro
Plasmid#240539PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-EF1α-nCas9/RT
Plasmid#210188PurposeExpression of nCas9/RT-P2A-PuroDepositorInsertnCas9/RT-P2A-Puro
UseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LMNA_mScarlet-I_Donor
Plasmid#178092PurposeHDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.DepositorInsertLMNA-mScarlet-I HDR donor
UseCRISPRExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-BlastR
Plasmid#196970PurposeLentiviral acceptor vector for pegRNAs with blasticidin resistanceDepositorTypeEmpty backboneUseCRISPRPromoterhU6Available SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-GsI-IIC-RT
Plasmid#181808PurposePE-GsI-IIC RTDepositorInsertPE-GsI-IIC-RT
UseCRISPRExpressionMammalianAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_VEGFA-mcherry
Plasmid#159790PurposeS. pyogenes sgRNA collocated with pegRNA targeting human VEGFA geneDepositorInsertspacer of sgRNA targeting VEGFA gene (VEGFA Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-GBA-10
Plasmid#199271PurposepegRNA used to induce GBA (c. 1226 A > G) mutationDepositorInsertGBA 1226AtoG pegRNA
ExpressionMammalianAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-ngRNA+14_EF1a-puroR
Plasmid#207359PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR +14 ngRNA
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G13A-tmpknot-epegRNA
Plasmid#214095PurposeLentiviral vector expressing epegRNA to induce KRAS G13A mutationDepositorInsertKRAS G13A epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET)
Plasmid#214083PurposeMammalian expression tetramerization domain (TET) of human TP53DepositorAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pvPE-V2
Plasmid#240286PurposeEncodes second generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.DepositorInsertpvPE-V2
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pvPE-V3
Plasmid#240287PurposeEncodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.DepositorInsertpvPE-V3
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_SRD5A1-mcherry
Plasmid#159780PurposeS. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 geneDepositorInsertspacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_EMX1-mcherry
Plasmid#159784PurposeS. pyogenes sgRNA collocated with pegRNA targeting human EMX1 geneDepositorInsertspacer of sgRNA targeting EMX1gene (EMX1 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_KCNA1-mcherry
Plasmid#159788PurposeS. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 geneDepositorInsertspacer of sgRNA targeting KCNA1 gene (KCNA1 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_SRD5A3-mcherry
Plasmid#159782PurposeS. pyogenes sgRNA collocated with pegRNA targeting human SRD5A3 geneDepositorInsertspacer of sgRNA targeting SRD5A3 gene (SRD5A3 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_HOXD13-mcherry
Plasmid#159793PurposeS. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 geneDepositorInsertspacer of sgRNA targeting HOXD13 gene (HOXD13 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLV-gag-hMLH1dn
Plasmid#195513PurposeMMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.DepositorInserthMLH1dn (MLH1 Human)
UseVirus like particleTagsFLAG, NES, and Protease cleavate siteExpressionMammalianMutationHuman MLH1 del754-756PromoterCMVAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G2_Dual_sgRNA
Plasmid#173201PurposeCoselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G2 sgRNA + user-specified sgRNA
UseCRISPRExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEmax_2star_pet21A
Plasmid#227675PurposeExpression plasmid for PEMAX** protein in bacteria.DepositorInsertPEmax_2_star
UseCRISPRTagsHis-tagExpressionBacterialPromoterT7Available SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA eBAR1-SV40-puro
Plasmid#240535PurposeLentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA-eBAR1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA-SV40-mCherry
Plasmid#240538PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.DepositorInsertepegRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-tmpknot-epegRNA
Plasmid#214093PurposeLentiviral vector expressing epegRNA to induce EGFR T790M mutationDepositorInsertEGFR T790M epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-GBA-N370S-tmpknot-epegRNA
Plasmid#214091PurposeLentiviral vector expressing epegRNA to induce GBA N370S mutationDepositorInsertGBA N370S epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_LMNA_CR-2-5
Plasmid#199275PurposepegRNA used to correct LMNA (c.1824C > T) mutationDepositorInsertLMNA 1824TtoC pegRNA
ExpressionMammalianAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only