Addgene

We narrowed to 2,944 results for: ARP

Showing: 201 - 220 of 2944 results

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_ARPC1B:1-372

Plasmid

#210905

Purpose

Insect expression of full-length human ARPC1B. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_ARPC1B:1-372

Plasmid

#211195

Purpose

Mammalian expression of full-length human ARPC1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ARPC1A:1-370

Plasmid

#211267

Purpose

Mammalian expression of full-length human ARPC1A. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1A:M1-M370 (ARPC1A Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ARPC1B:1-372

Plasmid

#211268

Purpose

Mammalian expression of full-length human ARPC1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-del.p119K120S–eGFP

Plasmid

#211554

Purpose

A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

eGFP

Expression

Mammalian

Mutation

deletion of amino acids 119K 120S

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLarp_N

Plasmid

#146982

Purpose

Insect Expression of DmLarp

Depositor

Elisa Izaurralde

Insert

DmLarp (larp Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutation N110T, P173L compared to …

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Use

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP

Plasmid

#185516

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

Replaces Q to P in GARQYNLI

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A870L

Plasmid

#174797

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A870L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A870L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-K893I

Plasmid

#174799

Purpose

Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-K893I (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

K893I, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hARPC2

Plasmid

#112549

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Li et al Sci Rep. 2017 Apr 19;7(1):915. doi: 10.1

Insert

ARPC2 (ARPC2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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ARPC4 C8.4 gRNA

Plasmid

#90533

Purpose

3rd generation lentiviral gRNA plasmid targeting human ARPC4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ARPC4 (Guide Designation C8.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ARPC4 C7.4 gRNA

Plasmid

#90532

Purpose

3rd generation lentiviral gRNA plasmid targeting human ARPC4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ARPC4 (Guide Designation C7.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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