We narrowed to 357 results for: RHOA

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GFP-mROCK1-delta7

Plasmid

#101295

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

727-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mNeongreen-pGBD(PKN1 codon optimized)

Plasmid

#129634

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding doamin of PKN1(30 - 100) codon optimized

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta5

Plasmid

#101293

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

375-727 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173865

Purpose

Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-HA/Net1 Δ1-121

Plasmid

#28263

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

2x HA

Expression

Mammalian

Mutation

Missing aa 1-121

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pLVUT-spC3GFP

Plasmid

#225487

Purpose

Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Secretable and permeable exoenzyme C3 transferase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 30-251

Promoter

Human ubiquitin C

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-MYH10_sgRNA

Plasmid

#183887

Purpose

pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Anillin

Plasmid

#68027

Purpose

Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Article

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (ANLN Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations at 798 5'-TGCC TCTTTGAATAAA…

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Lck-mCherry-RGS

Plasmid

#112935

Purpose

Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

Depositor

Dorus Gadella

Article

Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

Insert

p115RhoGEF-RGS domain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-MLC2 WT

Plasmid

#195235

Purpose

Mammalian expression of GFP tagged MLC2. Wild-type

Depositor

David Bryant, Keith Mostov

Article

Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

Insert

MLC2 (MYL2 Human)

Expression

Mammalian

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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lentiviral shRNA GEF-H1

Plasmid

#21477

Depositor

Richard Huganir

Article

Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rock1-GBD (840-110 aa) in pEGFPN1

Plasmid

#187279

Purpose

Express EGFP-Rock1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Rock1 (Rock1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

GBD (84-110aa)

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-CfANLN

Plasmid

#183837

Purpose

Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN homology arms with mNeonGreen-linker

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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