We narrowed to 5,490 results for: crispr cas9 grna plasmid

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pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

Plasmid

#171100

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Neurog2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

Plasmid

#159660

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

Plasmid

#159661

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

Plasmid

#159659

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

Plasmid

#167296

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAX (BAX Human)

Expression

Mammalian

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato SOX2-sgRNA

Plasmid

#196190

Purpose

Editing human SOX2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ANLN_sgRNA

Plasmid

#183876

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ANLN sgRNA spacer (ANLN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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