We narrowed to 7,283 results for: Ank

1. pECE M2-SNX17 WT

   Plasmid

   #69811

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CamKIIa:smFP-TVA-RGV

   Plasmid

   #202594

   Purpose

   Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

   Depositor

   Oleksandr Shcheglovitov

   Article

   Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

   Inserts

   smFP
   TVA
   RGV
   

   Use

   Lentiviral

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-Flag-Narg2

   Plasmid

   #49247

   Purpose

   mammalian expression of flag-tagged human Narg2

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Narg2 (NARG2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pFE-Selo-FI-prk

   Plasmid

   #162698

   Purpose

   Expresses S. elongatus Form IB rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   S. elongatus Form IB rubisco and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RS35075_pMQ30_KO_Kan

   Plasmid

   #185390

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS35075 upstream flanking homology region
   RR42_RS35075 downstream flanking homology region
   nptII kanamycin resistance marker
   

   Expression

   Bacterial

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Promoter

   yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. E. coli expression: Z-lock cofilin dark state mutant

   Plasmid

   #141135

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Bacterial

   Mutation

   D3 deletion, I32F in Zdk2

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23a-His-hRanGAP1tail

   Plasmid

   #53139

   Purpose

   E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   RanGAP1 (RANGAP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal fragment (amino acids 398-587)

   Promoter

   T7

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. THOC1

   Plasmid

   #156081

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   THOC1 (THOC1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAD16_CAD_12-490_pvp008

    Plasmid

    #124842

    Purpose

    Expresses Aspergillus terreus CAD in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-11, synthetic codon optimiz…

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. SR140

    Plasmid

    #156037

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SR140 (U2SURP Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178574

    Purpose

    Lentiviral vector for CamKIIα::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Promoter

    CaMKIIa

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter and under same promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV2/9-CW3SL-mCherry-DrTrkB

    Plasmid

    #184002

    Purpose

    AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    AAV

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCM

    Plasmid

    #162708

    Purpose

    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 MEKK4alpha WT

    Plasmid

    #12187

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

17. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178575

    Purpose

    Lentiviral vector for GFAP::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Promoter

    GFAP

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SYMPK

    Plasmid

    #156071

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SYMPK (SYMPK Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-flex-GFP-shRNA-scramble

    Plasmid

    #182503

    Purpose

    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Promoter

    hSyn promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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