Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,305 results for: LEA

Clear keywords
Showing: 2181 - 2200 of 12305 results

  1. pCAG roGFP2-mito

    Plasmid
    #168509
    Purpose
    Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential
    Depositor
    Franck Polleux
    Publication
    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-roGFP2
    Tags
    roGFP2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    168509-plasmid-map-sequence-id-329887

    Enlarge

  2. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  6. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

    Enlarge

  9. pGTvL1-SGC

    Plasmid
    #39188
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Tags
    GST and TEV cleavage site
    Expression
    Bacterial
    Promoter
    Tac (lactose/IPTG inducible)
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pBOB-synP-HT

    Plasmid
    #30456
    Depositor
    Edward Callaway
    Insert
    histone GFP
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Available since
    April 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    30456-plasmid-map-sequence-id-45568

    Enlarge

  14. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109428-plasmid-map-sequence-id-212894

    Enlarge

  15. pDS24

    Plasmid
    #58952
    Purpose
    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Publication
    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mitochondrially coded Arg8 protein
    Mutation
    Coding sequence synthetic in S. cerevisiae mitoch…
    Promoter
    n/a
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pGBW-m4046852

    Plasmid
    #145584
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145584-plasmid-map-sequence-id-292508

    Enlarge

  20. BAT-gal

    Plasmid
    #20889
    Depositor
    Stefano Piccolo
    Publication
    Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    7XTCF binding sites, Siamois minimal promoter, NLS-lacz
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    7 multimerized TCF-binding elements cloned upstre…
    Available since
    May 7, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2181 - 2200 of 12305 results