We narrowed to 15,788 results for: LEA
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Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
NET37 pmRFP-N2 (450)
Plasmid#61980Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET37 (KIAA1161 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE3987
Plasmid#137716PurposeExpresses dLbCas12a-RVRR-BE in mammalian cells.DepositorInsertdLbCas12a-RVRR-BE
UseTags6xHis, APOBEC-1, SV40 NLS, and UGIExpressionMammalianMutationG532A, K538V, Y542R, K595RPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAPBPL pEGFP-C3 (1765)
Plasmid#62055Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTAPBPL (TAPBPL Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T3-ZO-2-1st-PDZ-delta-NLS
Plasmid#27425DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationDeletion of NLS-Nuclear Localization Signal: Nucl…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG (CC→SS)-6MT
Plasmid#41739DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE2-TetO-Chim-Pchi-Q61A
Plasmid#231520PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 61 Q to A of the HMG d…PromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A/E64M
Plasmid#231521PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutations at position 61 Q to A and 64 E to…PromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBS-845
Plasmid#218000PurposeStrep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertStrep-tag II
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-846
Plasmid#218001Purpose3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsert3xFLAG-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-847
Plasmid#218002PurposeMyc-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertMyc-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorInsertkif13b (Kif13b Mouse)
UseTagsCleavable 6 x HIS tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only