We narrowed to 18,624 results for: Met

1. mCyRFP1-mMaroon

   Plasmid

   #84486

   Purpose

   Direct FRET pair Fusion mCyRFP1-mMaroon

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-mMaroon1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mOrange pHis-//

   Plasmid

   #53304

   Purpose

   Empty vector to express N-terminal His-mOrange-tagged fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Type

   Empty backbone

   Use

   Tags

   His and mOrange

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD/HisB-iRFP682

   Plasmid

   #45454

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP682
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

   Promoter

   araBAD

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD7

   Plasmid

   #73916

   Purpose

   gRNA vector for AsCpf1

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   tRNA:hAsCpf1-gRNA:tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. IRES-H2B-YFP-DD

   Plasmid

   #96893

   Purpose

   Reporter to monitor translation of EMCV IRES mRNA

   Depositor

   Tobias Meyer

   Article

   Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

   Insert

   IRES-H2B-Venus-DD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB1-T-Sapphire

   Plasmid

   #103977

   Purpose

   Plasmid encoding T-Sapphire

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   T-Sapphire
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (Q69M, C70V, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMVP-Cas9N

   Plasmid

   #80934

   Purpose

   Plasmid that expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIP-Rx3(A7)-mEGFP

   Plasmid

   #35531

   Depositor

   Robert Cohen

   Article

   Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

   Insert

   Rx3(A7)-mEGFP
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMVP-Cas9C

   Plasmid

   #80939

   Purpose

   Plasmid that expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DS-NMcas

    Plasmid

    #48646

    Purpose

    Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    NM tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    NM tracrRNA promoter and proC

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmTurquoise-N1

    Plasmid

    #60559

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

    Insert

    mTurquoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDS244

    Plasmid

    #34982

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Ste11 (STE11 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/HisB-PhoCl

    Plasmid

    #87688

    Purpose

    Express PhoCl in E. coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-EF1a-MegaGate-DD-Hygro

    Plasmid

    #175267

    Purpose

    MegaGate destination with C-terminal DD tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    Destabilization Domain

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPSmOrange-Tubulin

    Plasmid

    #31919

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKeratin-PSmOrange

    Plasmid

    #31924

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Keratin

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M-tdTom-SP

    Plasmid

    #48677

    Purpose

    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Sp6

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pEB1-mTurquoise2

    Plasmid

    #103974

    Purpose

    Plasmid encoding mTurquoise2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mTurquoise2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEB1-mTurquoise

    Plasmid

    #103972

    Purpose

    Plasmid encoding mTurquoise

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mTurquoise
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge