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We narrowed to 10,323 results for: Coli

Showing: 4401 - 4440 of 10323 results
  1. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pKP1693

    Plasmid
    #167595
    Purpose
    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Insert
    FepA
    Expression
    Bacterial
    Mutation
    His6 tag inserted after residue 393 in the mature…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    CCL20 (CCL20 Synthetic, Escherichia coli, Human)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pMRBad-C-wtBlc

    Plasmid
    #168257
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly
    Depositor
    Insert
    C-fragment of wtBlc
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pJM108

    Plasmid
    #134160
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Insert
    Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    KMLL, E. coli codon optimized, CRISPR/Cas9 harden…
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pJM107

    Plasmid
    #134159
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Insert
    Nde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pLY151

    Plasmid
    #130947
    Purpose
    pspFΔHTH::λN22plus gene controlled by promoter PrhaB
    Depositor
    Inserts
    rhaS
    pspFΔHTH::λN22plus
    Use
    Synthetic Biology
    Tags
    pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    The HTH domain of the pspF gene was deleted (297-…
    Promoter
    Pcon and PrhaB
    Available Since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pAL832

    Plasmid
    #119305
    Purpose
    precusor outer membrane protein A, ompA, truncated after amino acid 176
    Depositor
    Insert
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    Tags
    none
    Expression
    Bacterial
    Mutation
    truncated after amino acid 176, mutations Threoni…
    Promoter
    lac
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pBP-J23108

    Plasmid
    #72964
    Purpose
    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Insert
    J23108 promoter
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pBP-J23114

    Plasmid
    #72965
    Purpose
    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Insert
    J23114 promoter
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pBP-T7_RBS

    Plasmid
    #72978
    Purpose
    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)
    Depositor
    Insert
    T7+RBS
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  14. pBP-Strep(II)

    Plasmid
    #72984
    Purpose
    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Insert
    Strep(II) N-terminal tag
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. pTU2-A-lacZ

    Plasmid
    #72950
    Purpose
    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Insert
    Level 2 - A site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pTU2-B-lacZ

    Plasmid
    #72951
    Purpose
    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Insert
    Level 2 - B site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4401 - 4440 of 10323 results