We narrowed to 30,779 results for: Ide

1. CRY2-INPP5E

   Plasmid

   #79562

   Purpose

   expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

2. CRY2-INPP5E(D556A)

   Plasmid

   #79564

   Purpose

   expression of CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pFLAG-EEF1D (pcDNA 3.1+) LG3-1

   Plasmid

   #37364

   Depositor

   David Litchfield

   Article

   Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

   Insert

   EEF1D (EEF1D Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCOA-Leu-FvPPT1-fsr1

   Plasmid

   #179868

   Purpose

   Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Inserts

   PPT1
   fsr1
   

   Expression

   Yeast

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Promoter

   Yarrowia lipolytica EXP1 and Yarrowia lipolytica …

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-CRY2-INPP5E

   Plasmid

   #79561

   Purpose

   expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-CRY2-INPP5E(D556A)

   Plasmid

   #79563

   Purpose

   expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCJ217

   Plasmid

   #162685

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   G489C, S530C; codon optimized for E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-mFzd8

   Plasmid

   #102870

   Purpose

   Expresses mouse Fzd8 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Frizzled 8 (Fzd8 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T to C substitution at nucleotide 1134, C to T su…

   Promoter

   EF1A

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ200

   Plasmid

   #162673

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C and a sequence Y75-F104 to introduce a 6th…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK034

    Plasmid

    #72383

    Purpose

    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Insert

    phosphoribosylglycinamide synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Yeast Secrete and Detect

    Plasmid Kit

    #1000000166

    Purpose

    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

    Depositor

    Sylvestre Marillonnet

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Start-Stop Assembly Toolkit

    Plasmid Kit

    #1000000152

    Purpose

    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.

    Depositor

    John Heap

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-RupA_T1-NHis

    Plasmid

    #109259

    Purpose

    NRPS T domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T1
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 917..1025 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-RupA_R-NHis

    Plasmid

    #109257

    Purpose

    NRPS R domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_R
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 2038..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJQ210SK

    Plasmid

    #78499

    Purpose

    Cosmid version of suicide vector pJQ200SK. Suicide vector

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector

    Tags

    none

    Promoter

    non

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

17. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available Since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pET29b-RupA_T2-R-CHis

    Plasmid

    #109258

    Purpose

    NRPS di-domain containing TR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T2-R
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1939..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-RupA_C2-A2-T2-R-CHis

    Plasmid

    #109256

    Purpose

    NRPS module containing CATR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C2-A2-T2-R
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1023..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-RupA_C1-A1-T1-CHis

    Plasmid

    #109255

    Purpose

    NRPS module containing CAT domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C1-A1-T1
    

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1..1025 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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