175,825 results
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Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
TagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AVPR1A-DuET
Plasmid#213186PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AVPR1B-DuET
Plasmid#213187PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP
Plasmid#10676PurposeRetroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAi and RetroviralExpressionMammalianAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#106895PurposeExpresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianPromoterCAG and PGKAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAP1-2
Plasmid#125838PurposeKO BAP1 geneDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt2
Plasmid#66831PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.flex.H2B.GFP
Plasmid#170368PurposeCre-inducible AAV vector expressing nuclear GFP.DepositorInsertflex H2B GFP
UseAAVPromoterhuman SynapsinAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIIS928
Plasmid#238211PurposeExpresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.DepositorInsertTaTasR
TagsHA-NLSExpressionMammalianAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only