We narrowed to 14,080 results for: UNG

1. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFC330

   Plasmid

   #87842

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

   Plasmid

   #173461

   Purpose

   C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-I-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 nickase for Split-PE (pJUL2497)

   Plasmid

   #190104

   Purpose

   Expresses nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   Tags

   XTEN-bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIP-AMPKa1_WT

   Plasmid

   #79010

   Purpose

   For mammalian expression of WT AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-pMagHigh-FlpC

   Plasmid

   #180586

   Purpose

   PA-Flp

   Depositor

   Won Do Heo

   Article

   Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

   Insert

   Split FlpO
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HERV-Kcon-RT for Split-PE (pJUL3120)

   Plasmid

   #190107

   Purpose

   Expresses HERV-Kcon RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   HERV-Kcon-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYSD085

    Plasmid

    #212922

    Purpose

    Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    649 Stalk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFPL-Gg

    Plasmid

    #61653

    Purpose

    Gateway vector for generating green fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL7044

    Plasmid

    #167215

    Purpose

    overexpression of PbrR in E. coli for cell-free lead sensing

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    PbrR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBL7061

    Plasmid

    #167230

    Purpose

    cell-free cascaded lead sensor

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pPbr-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-SERT-myc

    Plasmid

    #107456

    Purpose

    Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG426GPD-ccdB-yeastSMASh-YFP

    Plasmid

    #68854

    Purpose

    Expresses SMASh tag fused to YFP at N terminus in yeast

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    yeastSMASh-YFP
    

    Use

    Tags

    SMASh tag

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIP-caTfeb

    Plasmid

    #79013

    Purpose

    For mammalian expression of a shortened, constitutively active version of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (TFEB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removed first ~400 bp from 5' end (N-terminu…

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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