We narrowed to 160,365 results for: des
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Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Snaptag-RfA
Plasmid#186385PurposeDestination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727A-STAT3-3xFlag
Plasmid#99262PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727A)DepositorInsertSignal transducer and activator of transcription 3 - S727A (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationS727APromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTR AE Clover
Plasmid#90240PurposeLentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (AE mutant)
UseLentiviralTagsmCloverExpressionMammalianPromoterPGKAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-cjBLIMP1-Venus-HygTK
Plasmid#141028PurposeReporter integrationDepositorInsertmVenus
ExpressionMammalianAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectPromotermetallothioneinAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727D-STAT3-3xFlag
Plasmid#99263PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727D)DepositorInsertSignal transducer and activator of transcription 3 - S727D (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationS727DPromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B2-GFP
Plasmid#123109PurposeExpresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
Tags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-emiRFP703-stop
Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES
Plasmid#85750PurposeExpresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2DepositorInsertsType II-C anti-CRISPR AcrIIC3Nme
mTagBFP2
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
IZ401: pMVP/Lenti/Neo-DEST
Plasmid#121850PurposepMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS481-pCDest2-AcrE2-mTagBFP2-IRES
Plasmid#85749PurposeExpresses anti-CRISPR protein from Type I-E and mTagBFP2DepositorInsertsType I-E anti-CRISPR
mTagBFP2
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-Y705F-STAT3-3xFlag
Plasmid#99261PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (Y705F)DepositorInsertSignal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationY705F (contains a silent mutation: nucleotide 271…PromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B G120
Plasmid#123116PurposeExpresses EGFP-LC3B G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFPExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P1-4A
Plasmid#51252PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 789,805,817,848 mutated to AlaPromoterEF1aAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(92Q)-S776D
Plasmid#21757DepositorInsertAtaxin-1 (ATXN1 Human)
TagsGSTExpressionMammalianMutationinsert contains a stretch of 92 Q(Glutamines) and…PromoterCMVAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
Plasmid#56658Purposeexpresses constitutively active human PKCi with a deletion on the PB1 domainDepositorInsertHuman PKCi (PRKCI Human)
UseLentiviralTagsV5/HisMutationA120E and deletion of PB1 domain (aa 24–98)PromoterCMVAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#118725PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only