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OeNL_pRSETb

Plasmid

#89529

Purpose

Orange color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Publication

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Publication

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDONOR223 RpH-LAMP1-3xFLAG

Plasmid

#163019

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Publication

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-K289E

Plasmid

#30380

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN-K289E (PTEN Human)

Expression

Mammalian

Mutation

K289E

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Cyto-mTurq2-Apollo-NADP+

Plasmid

#193301

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

Depositor

Jonathan Rocheleau

Publication

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G6PD (G6PD Human)

Tags

mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCD4-TagRFP-T (RHPMP151)

Plasmid

#119238

Purpose

Expresses CD4 fused to TagRFP-T

Depositor

Ricardo Henriques

Publication

Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD4 (CD4 Human)

Tags

tagRFP-T

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LSSmOrange

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LSSmOrange

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI

Plasmid

#160423

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Publication

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight3.8

Plasmid

#257855

Purpose

Expression of the green genetically encoded dopamine sensor dLight3.8 under synapsin promoter.

Depositor

Lin Tian

Publication

Roshgadol et al Res Sq [Preprint]. 2025 Aug 20:rs.3.rs-7

Insert

dLight3.8

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE37C

Plasmid

#129312

Purpose

ATP measurement

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-Hph

Plasmid

#188741

Purpose

pUC19 plasmid (MCS intact) with Hygromycin selection marker

Depositor

Michael Bromley

Publication

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

Hygromycin

Use

Filamentous fungal expression

Promoter

gpdA

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-jGCaMP7b-XN

Plasmid

#178362

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

Depositor

Xiaodong Liu

Publication

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

jGCaMP7b-XN

Expression

Mammalian

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM15B_WT_V5

Plasmid

#83010

Purpose

Gateway Donor vector containing RBM15B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM15B (RBM15B Human)

Use

Gateway Cloning

Mutation

R593K; p.1_327deleted

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3_QLuc

Plasmid

#183260

Purpose

Express QLuc in mammalian cells

Depositor

Huiwang Ai

Publication

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

QLuc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Publication

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 -5'cMyc-SMCl- S957A

Plasmid

#32368

Depositor

Michael Kastan

Publication

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S957A

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only