We narrowed to 2,291 results for: Tub;

1. pRK5-EGFP-Tau P301L

   Plasmid

   #46908

   Purpose

   expresses EGFP tagged Tau P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau P301L (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV mCherry-GFP-LC3B G120A

   Plasmid

   #123235

   Purpose

   Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mScarlet-MAP4-MTBD

   Plasmid

   #171488

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mScarlet

   Expression

   Mutation

   Aminoacids 658-1126

   Promoter

   T7

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Puro GFP-LC3B G120

   Plasmid

   #123241

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Promoter

   PGK

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-miRFP-MAP4-MTBD

   Plasmid

   #171487

   Purpose

   T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   miRFP

   Expression

   Mutation

   Aminoacids 658-1126

   Promoter

   T7

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-EGFP MASTL WT (siRNA resistant)

   Plasmid

   #191011

   Purpose

   Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mIFP-MAPTau-N-10

   Plasmid

   #56230

   Purpose

   Localization: Microtubules, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   MAPTau (MAPT Human)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLenti PGK Puro GFP-LC3B G120A

   Plasmid

   #123242

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   PGK

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pROF346

   Plasmid

   #155331

   Purpose

   Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid

    #207794

    Purpose

    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mCardinal-MCAK-C-7

    Plasmid

    #56168

    Purpose

    Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    MCAK (KIF2C Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pDEST-CMV 3xFLAG-LC3B-G120A-GFP

    Plasmid

    #123108

    Purpose

    Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK EGFP-LC3B

    Plasmid

    #200427

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2CG NBT:NRGIIIa_R551Q:polyA

    Plasmid

    #193013

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa R551Q (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-EGFP MASTL G44S (siRNA resistant)

    Plasmid

    #191012

    Purpose

    Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G44S

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUb FLAG-Mts

    Plasmid

    #195065

    Purpose

    Expresses FLAG-tagged Drosophila mts protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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