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Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorInsertAMPK Biosensor
UseTagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterC. neoformans U6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-ZO-2
Plasmid#27422DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H2
Plasmid#79513PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDSDepositorInsertLXR BETA (NR1H2 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
sh-RNA hAtg13
Plasmid#31971DepositorInsertAtg13 (ATG13 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 Flag TSC2 (S939A/T1462A)
Plasmid#14131DepositorInsertTSC2 S939A/T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A/T1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
Plasmid#159859PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallin and cmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-DEST
Plasmid#106348PurposeDestination vector for gateway cloning. Developed for Flp-In T-REx system.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
UseTagsLyn kinase sequence (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SUV39h1 ts2
Plasmid#115881PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME18s-HA-hFIP200
Plasmid#24303DepositorInsertFIP200 (RB1CC1 Human)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SUV39h1 ts1
Plasmid#115880PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDicer
Plasmid#89144PurposeExpression of hDicer in Sf9 CellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
C3 luc (T1 del)
Plasmid#11358DepositorInsertC3 promoter (C3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mitoT
Plasmid#174519PurposeEngineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cellsDepositorInsertMitoT synthetic tether
UseTags6xHis and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A)
Plasmid#14132DepositorInsertTSC2 S939A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A.PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1A eIF4A3
Plasmid#83035Purposemammalian expression of eIF4A3DepositorInserteIF4A3 (EIF4A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS2-V5 puro
Plasmid#158642PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS2.DepositorInsertOAS2 (OAS2 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorInsertTSC2 T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationT1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti c-MYC-DDK-Puro GFP
Plasmid#123299PurposeMammalian expression of GFP C-MYC-FLAGDepositorInsertGFP
UseLentiviralTagsMYC-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6B
Plasmid#19884DepositorInserttrinucleotide repeat containing 6B (TNRC6B Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
L1_lacZgRNA-Ck3
Plasmid#136136PurposeL1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screeningDepositorInsertp5-MpU6:lacZgRNA
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
L1_Cas9-Ck4
Plasmid#136135PurposeL1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectorsDepositorInsertp5-MpEF1a:Cas9_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Ash2L full
Plasmid#15549DepositorInsertAsh2L (ASH2L Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-MCU-Flag
Plasmid#50054PurposeExpression of human MCU in mammalian cellsDepositorInsertMCU-Flag (MCU Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
L1_lacZgRNA-Ck2
Plasmid#136137PurposeL1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screeningDepositorInsertp5-MpU6:lacZgRNA
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4A3_I
Plasmid#146581PurposeMammalian Expression of HseIF4A3DepositorInsertHseIF4A3 (EIF4A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F6
Plasmid#37965DepositorInsertE2F6 (E2F6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
UseTagsIntein-Chitin-binding domainExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVenusNfN
Plasmid#128564PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only