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6xABRE_A

Plasmid

#185803

Purpose

A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(ABI1)+-86MP+erGFP

Expression

Plant

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_R

Plasmid

#185804

Purpose

a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(RD29A)+-86MP+erGFP

Expression

Plant

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204786

Purpose

Bacterial expression of Sars Cov 2 MPRO T190I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

T190I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204784

Purpose

Bacterial expression of Sars Cov 2 MPRO Q189K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

Q189K

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204783

Purpose

Bacterial expression of Sars Cov 2 MPRO P132H mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

P132H

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204782

Purpose

Bacterial expression of Sars Cov 2 MPRO N142S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

N142S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

G143S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204774

Purpose

Bacterial expression of Sars Cov 2 MPRO A191V mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191V

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204773

Purpose

Bacterial expression of Sars Cov 2 MPRO A193T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204772

Purpose

Bacterial expression of Sars Cov 2 MPRO A191T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A191T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204771

Purpose

Bacterial expression of Sars Cov 2 MPRO A193S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

A193S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGTag Hygro BFP nLAP +0

Plasmid

#194317

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP -1

Plasmid

#194324

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP -0

Plasmid

#194323

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP -2

Plasmid

#194325

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +1

Plasmid

#194318

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +2

Plasmid

#194319

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6MYC

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UPATrap-VC-ORF2

Plasmid

#110653

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UPATrap-VC-ORF1

Plasmid

#110652

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UPATrap-VC-ORF0

Plasmid

#110651

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CDS_mTurqcp_chlo

Plasmid

#163936

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

FP optimised for Marchantia polymorpha chloroplast expression, from Genbank ID KT364744

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p663-UBC-miniTurbo-V5-STK32B_IDG-K

Plasmid

#170609

Purpose

Gateway destination clone of STK32B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-STK32B (STK32B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTR235syn

Plasmid

#133464

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR235

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR400syn

Plasmid

#133465

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR400

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR625syn

Plasmid

#133466

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR625

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR060syn

Plasmid

#133462

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR060

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR120syn

Plasmid

#133463

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR120

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMM0626

Plasmid

#129001

Purpose

pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0622

Plasmid

#128997

Purpose

pTEF1-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0627

Plasmid

#129002

Purpose

pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0658

Plasmid

#129019

Purpose

pREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0657

Plasmid

#129018

Purpose

pRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0656

Plasmid

#129017

Purpose

pTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0629

Plasmid

#129004

Purpose

pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0620

Plasmid

#128995

Purpose

pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0628

Plasmid

#129003

Purpose

pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.284

Plasmid

#119650

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS1 DOWN Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.283

Plasmid

#119649

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS1 UP Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.282

Plasmid

#119648

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS2 DOWN Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.281

Plasmid

#119647

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS2 UP Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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