We narrowed to 4,891 results for: Mela

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pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pRK5_tAKARδ

Plasmid

#119916

Purpose

Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARδ

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARε

Plasmid

#119917

Purpose

Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARε

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only
Lenti_CRISPRi_sgNeg1

Plasmid

#166998

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PRB

Plasmid

#89130

Purpose

mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + codon optimized Brn3a

Plasmid

#86829

Purpose

mammalian expression of the transcription factor Brn3a

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

Brn3a (POU4F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-flex-plap

Plasmid

#80422

Purpose

tracing neuronal projection

Depositor

Nirao Shah

Article

Delwig et al PLoS One. 2016 Feb 19;11(2):e0149501. do

Insert

plap (ALPP Human)

Use

AAV

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-NLS-AcrIIA4

Plasmid

#113037

Purpose

Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SV40 NLS-AcrIIA4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4

Plasmid

#29527

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

Wild Type

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCHA1.1-MFSD12-V5

Plasmid

#149508

Purpose

Stable expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Lentiviral

Tags

V5

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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