We narrowed to 9,260 results for: Pol

1. pL0_CD-mcherry

   Plasmid

   #202143

   Purpose

   Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   mCherry gene
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL0_CD-eCFP

   Plasmid

   #202142

   Purpose

   Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   eCFP gene
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL0_CD-roGFP

   Plasmid

   #202140

   Purpose

   Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   roGFP gene
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL0_CD-eYFP

   Plasmid

   #202139

   Purpose

   Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   eYFP gene
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL0_AF-spacer

   Plasmid

   #202130

   Purpose

   Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Spacer
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGAL1-VP1 (LEU2)

   Plasmid

   #182480

   Purpose

   Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Insert

   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRuby3 only VLP

   Plasmid

   #182429

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-mRuby3
   deltaVP1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-PC(1-86)-H6

   Plasmid

   #183780

   Purpose

   Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. L1-Ck1-hs2-l-chlo

   Plasmid

   #163938

   Purpose

   For integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha rbcL_trnR region homologous sequence
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176577

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6637

    Plasmid

    #106164

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGWB434

    Plasmid

    #68699

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Promoter

    MpHSP17.8A1 promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGWB433

    Plasmid

    #68698

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Citrine

    Expression

    Plant

    Promoter

    MpHSP17.8A1 promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGWB410

    Plasmid

    #68675

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGWB413

    Plasmid

    #68678

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMpGWB233

    Plasmid

    #68624

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Citrine

    Expression

    Plant

    Promoter

    MpHSP17.8A1 promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGWB210

    Plasmid

    #68601

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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