We narrowed to 10,582 results for: plasmids 101

1. PS-CFP2-Keratin-17

   Plasmid

   #57233

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   NM_199187.1 (KRT18 Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. PA-mCherry1-Keratin-17

   Plasmid

   #57184

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. mGeos2-VEL-Keratin-17

   Plasmid

   #57550

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 503 / 569, Emission: 514 / 581

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mGeos2-VEL

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. CAMK2D

   Plasmid

   #38904

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2D (CAMK2D Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. CAMK2D

   Plasmid

   #38987

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2D (CAMK2D Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. MYST1

   Plasmid

   #25180

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MYST1_01 (KAT8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. DMPK

   Plasmid

   #38892

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DMPK (DMPK Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. ARL10B:L9-K182

   Plasmid

   #25523

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ARL10B:L9-K182 (ARL8A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids L9-K182

   Promoter

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Use

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available Since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119945

    Purpose

    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW57.1_SPTLC1_WT

    Plasmid

    #181920

    Purpose

    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

    Depositor

    Christian Metallo

    Article

    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

    Insert

    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    

    Use

    Lentiviral; Mammalian expression, , doxycycline i…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP109 pEF1a-gRNA-GFP

    Plasmid

    #100935

    Purpose

    GFP, puromycin and gRNA scaffold driven by EF1a promoter

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA scaffold
    

    Use

    Grna scaffold

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCV PIG C/EBPa

    Plasmid

    #66985

    Purpose

    Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP alpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

    Plasmid

    #127865

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    deleted the c-terminal 20 amino acids

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX nsp1 K164A/H165A CoV2

    Plasmid

    #175514

    Purpose

    For protein expression of K164A/H165A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    K164A/H165A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX nsp1 R124A/K125A CoV2

    Plasmid

    #175513

    Purpose

    For protein expression of R124A/K125A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R124A/K125A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX C_K nsp1 K164A/H165A CoV2

    Plasmid

    #175517

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge