We narrowed to 7,670 results for: URE

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAcrF1

Plasmid

#89233

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF1

Tags

6xHis-TEV and FLAG

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSK24

Plasmid

#72852

Purpose

template for C-terminal Twin-Strep tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

twin Strep tag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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SaDHFR-pNIC-Bsa4

Plasmid

#231039

Purpose

Expresses Staphylococcus aureus Dihydrofolate reductase under T7 with HIS tag

Depositor

Josh Beckham

Insert

SaDHFR

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-G

Plasmid

#216115

Purpose

Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

oROS-G

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_pLyn_EGFP

Plasmid

#214605

Purpose

Expresses fluorescent protein EGFP targeted to plasma membrane

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

lyn-EGFP

Tags

lyn targetting

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_ABE_D3_empty

Plasmid

#203809

Purpose

Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

ABE_D3

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβ

Plasmid

#187361

Purpose

Lentiviral expression of GPa3b17 hTCR beta

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRα

Plasmid

#187360

Purpose

Lentiviral expression of GPa3b17 hTCR alpha

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_EPHA2

Plasmid

#183286

Purpose

All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

EPHA2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ERBB2

Plasmid

#183287

Purpose

All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ERBB2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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p045

Plasmid

#70057

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with pDC184

Depositor

Axel Brunger

Article

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

C2AB-linker-SNAP-25_C
SNAP-25_N

Tags

No tags

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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GW1-ARSeNL

Plasmid

#207873

Purpose

Mammalian expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pF-N-HiBiT-template

Plasmid

#200882

Purpose

template for N-terminal HiBIT-tagging

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-linker

Expression

Mammalian

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.EF1a.FLEX.MTRIA-OT

Plasmid

#184595

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human elongation factor-1 alpha promoter

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway Cloning

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFX21

Plasmid

#44533

Depositor

Michael Grunstein

Article

Xu et al Mol Cell. 2007 Sep 21;27(6):890-900.

Insert

SIR2-6xHis

Tags

His6

Expression

Bacterial

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
xGoosecoid

Plasmid

#24977

Depositor

Edward De Robertis

Article

Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

Insert

Goosecoid (gsc-a Frog)

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only