We narrowed to 11,729 results for: pcDNA

1. pcDNA-HA-Nedd4/2795

   Plasmid

   #11426

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D661N compared to GenBank reference sequence U966…

   Promoter

   CMV

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CaMPARI V398D

   Plasmid

   #60786

   Purpose

   Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI V398D
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   V398D

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 MLL2 653

   Plasmid

   #11017

   Depositor

   Matthew Meyerson

   Article

   Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

   Insert

   MLL2 (KMT2B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   truncation of MLL2, last 653 amino acids, from pr…

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
   

   Use

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-OTI-TCRB

   Plasmid

   #131036

   Purpose

   Expresses OT-I TCRB

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Insert

   TCRB (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4.TO-ORF52-2xCSTREP

   Plasmid

   #136212

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF52
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3puro-mGFP-TOP3B

   Plasmid

   #225470

   Purpose

   Express mEGFP-fusion protein of TOP3B

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   TOP3B (TOP3B Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Akt-Scramble

   Plasmid

   #175014

   Purpose

   Negative-control construct for Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-Scramble
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1.Gα11-LgB97

    Plasmid

    #134361

    Purpose

    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα11-LgB97 (GNA11 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-myc-Ataxin3Q84

    Plasmid

    #22124

    Depositor

    Henry Paulson

    Article

    Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(+)-iBB-GECO1

    Plasmid

    #194215

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

    Depositor

    Jin Zhang

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Mcm5-mCer

    Plasmid

    #211393

    Purpose

    mammalian expression of human Mcm5 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    Mcm5 (MCM5 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

    Plasmid

    #50030

    Purpose

    Expression of cytosolic S. pyogenes SrtA in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-HshnRNPR_AA

    Plasmid

    #148235

    Purpose

    Mammalian Expression of HshnRNPR

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPR (HNRNPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ccdB-TwinStrep

    Plasmid

    #214988

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-hTLR3-VenusFlag

    Plasmid

    #197636

    Purpose

    Human CD283 protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TLR3 (TLR3 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-His-SUMO3

    Plasmid

    #159325

    Purpose

    Mammalian cell expression of mouse His-SUMO3

    Depositor

    Jianjun Zhao

    Insert

    His-SUMO3 (Sumo3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge