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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,788 results for: plat

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Showing: 2281 - 2300 of 8788 results

  2. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    James Chappell
    Publication
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174369-plasmid-map-sequence-id-345702

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  4. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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  15. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Publication
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  16. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

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  18. pJUMP18-lambdaRED_NOC

    Plasmid
    #127032
    Purpose
    Basic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).
    Depositor
    Chris French
    Publication
    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00
    Insert
    Part ?RED_NOC
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Available since
    July 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    127032-plasmid-map-sequence-id-252074

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  19. pMaCTag-Z03

    Plasmid
    #120046
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    moxCerulean3
    Use
    Pcr template
    Tags
    moxCerulean3
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120046-plasmid-map-sequence-id-232652

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  20. pMaCTag-P10

    Plasmid
    #120021
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKOk
    Use
    Pcr template
    Tags
    mKOk
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120021-plasmid-map-sequence-id-232619

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Showing: 2281 - 2300 of 8788 results