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pMC-1-TBS-11

Plasmid

#197689

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

TBS enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-EGFP-9

Plasmid

#197733

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EGFP

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2-Reverse

Plasmid

#197801

Purpose

Level 1 Module 2 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDREV-1

Plasmid

#26751

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

H2B-Venus CDS (H2BC3 Human)

Use

Cre/Lox; Flp/frt; dre/rox

Tags

Venus

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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MSGV Rh Acceptor PGK-NGFR

Plasmid

#64272

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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YeNL-Actin_pcDNA3

Plasmid

#89521

Purpose

Yellow color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-CeNL_pcDNA3

Plasmid

#89515

Purpose

Cyan color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFLIP37

Plasmid

#65505

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-Cer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-18

Plasmid

#119997

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA HA HP1 gamma delta C

Plasmid

#24081

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

HA

Expression

Mammalian

Mutation

Missing C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1

Plasmid

#79912

Purpose

piggyBac transposon vector, Cerulean-2A-sHRPbNLG1

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Cerulean-2A-sHRPbNLG1

Use

Piggybac

Expression

Mammalian

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

Plasmid

#79913

Purpose

piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Zeocin-t2A-sHRPbNRX3beta

Use

Piggybac

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-01

Plasmid

#119980

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-09

Plasmid

#119988

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x6

Plasmid

#63600

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-29

Plasmid

#120008

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-04

Plasmid

#119983

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRS316Nab3

Plasmid

#110533

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSR270

Plasmid

#14057

Depositor

Richard Hynes

Article

Schwarzbauer et al Cell. 1983 Dec . 35(2 Pt 1):421-31.

Insert

Rat Fibronectin (Fn1 Rat)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBS-tWnt5a

Plasmid

#169372

Purpose

Expresses a part of Chinese soft-shell turtle Wnt5a for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt5a

Expression

Bacterial

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-tWnt7b

Plasmid

#169371

Purpose

Expresses a part of Chinese soft-shell turtle Wnt7b for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt7b

Expression

Bacterial

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-08

Plasmid

#119987

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha1R

Plasmid

#68226

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha2R

Plasmid

#68227

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge