We narrowed to 29,245 results for: pET

1. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Vitiosangium-bGSDM-thrombin

   Plasmid

   #222099

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Vitiosangium-bGSDM-thrombin ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Nostoc-bGSDM-WELQ

   Plasmid

   #222100

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Nostoc-bGSDM-WELQ ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23b-Casp7-His

   Plasmid

   #11825

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 7 (CASP7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-32a(+)-TGFB1

   Plasmid

   #120361

   Purpose

   Produce human recombinant TGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β1 (TGFB1 Synthetic, Human)
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a-link-NGFP

   Plasmid

   #52732

   Purpose

   IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   link-NGFP
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a Bm-iPGM-bio

    Plasmid

    #162565

    Purpose

    Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    B. malayi iPGM bio-6His
    

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28C-mCherry-HNRNPA1LCD

    Plasmid

    #204405

    Purpose

    E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    HNRNPA1 (186-320) (HNRNPA1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-29b(+)-mSandy2

    Plasmid

    #177760

    Purpose

    Expresses mSandy2 using the T7 Promoter

    Depositor

    Roberto Chica

    Article

    Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

    Insert

    mSandy2
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-mH6-Cas12c2

    Plasmid

    #120873

    Purpose

    Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Available Since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-11-LECT2-I40

    Plasmid

    #183032

    Purpose

    Expression of LECT2 I40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Expression

    Bacterial

    Mutation

    I40 variant

    Promoter

    T7

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET16b-MYPT1(1-314)

    Plasmid

    #224893

    Purpose

    Bacterial expression of MYPT1(1-314)

    Depositor

    Mathieu Bollen

    Article

    Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

    Insert

    myosin phosphatase targeting subunit 1 (PPP1R12A )
    

    Expression

    Bacterial

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21b-Caspase-3(C163S)

    Plasmid

    #90088

    Purpose

    Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C163S

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-TEV-TBP6.9F34MF37MF77M

    Plasmid

    #168268

    Purpose

    Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    TBP6.9
    

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F34M, F37M and F77M

    Promoter

    T7 promotor

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28C-mCherry-53BP1LCD

    Plasmid

    #204407

    Purpose

    E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    53BP1(1208-1977) (TP53BP1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28b-miniE-DBCO

    Plasmid

    #181985

    Purpose

    Used to label proteins with the MiniR genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniE-DBCO
    

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. BARD1 26-327 pET28a

    Plasmid

    #12646

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET-His-Dre

    Plasmid

    #123130

    Purpose

    Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Dre recombinase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28a-LgBiT-SpyCatcher

    Plasmid

    #223634

    Purpose

    Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Tags

    His6, LgBiT

    Expression

    Bacterial

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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