Addgene

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GAL 25Q+ProCFPp303

Plasmid

#15574

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt25Q + Pro (HTT Human)

Use

Tags

CFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1 1-153-YFP

Plasmid

#18690

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 1-153

Use

Tags

YFP

Expression

Yeast

Mutation

NonPrion domain of Rnq1

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
GAL HA25QDProCFPp303

Plasmid

#15578

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL HA103QDProCFPp303

Plasmid

#15579

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Tags

FLAG/HA and His6

Expression

Mutation

delta 657 (stop codon)

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pFUS_A3A

Plasmid

#43851

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Use

Tags

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Use

Tags

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pFUS_A4B

Plasmid

#43854

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pSR21

Plasmid

#69156

Purpose

read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
MET FLAG46QDProCFPp303

Plasmid

#15589

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Use

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL HA25Q+ProCFPp303

Plasmid

#15570

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Use

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
P1909:EVA4_RHISA-StrepII-8xHis

Plasmid

#233698

Purpose

Expresses Strep and His-tagged Evasin 4 (EVA4_RHISA) in mammalian cells

Depositor

Shoumo Bhattacharya

Article

Kryukova et al Commun Biol. 2025 Apr 3;8(1):549. doi: 1

Insert

EVA4

Use

Tags

Strep II, 8xHis

Expression

Mammalian

Mutation

Promoter

Chicken β actin

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1559

Plasmid

#218590

Purpose

Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Use

Tags

ePTS1

Expression

Yeast

Mutation

Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-HMF-LST2

Plasmid

#220786

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAceBAC2-MLG-HMF-LST2

Plasmid

#220787

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Insect

Mutation

Promoter

p10

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_CD28ICD

Plasmid

#197098

Purpose

This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of CD28.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pmcb306-GFP-Rab12-mito

Plasmid

#207349

Purpose

Mammalian expression of Rab12-mito

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab12-mito (RAB12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T58-Cterminal-mEmerald

Plasmid

#202353

Purpose

Talin(DeltaR4-10)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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