We narrowed to 6,241 results for: bre

1. dSpyCas9-p300-P2A-CASANOVA

   Plasmid

   #113129

   Purpose

   Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   dCas9-p300-P2A-CASANOVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG5-HA-ERR3 S45A

   Plasmid

   #37849

   Depositor

   Rebecca Riggins

   Article

   Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

   Insert

   Estrogen-related receptor gamma (Esrrg Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Serine 45 to Alanine (AGC to GCC)

   Promoter

   SV40

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti Lifeact-EGFP BlastR

   Plasmid

   #84383

   Purpose

   Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX317-EGFP

   Plasmid

   #115439

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAMP1-miRFP703

   Plasmid

   #79998

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   miRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. N-BLa 1.2

   Plasmid

   #88999

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti Lifeact-iRFP670 BlastR

   Plasmid

   #84385

   Purpose

   Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMYC-GFP

   Plasmid

   #42142

   Depositor

   Olufunmilayo Olopade

   Article

   Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-V5-KDM3A

    Plasmid

    #82331

    Purpose

    lentiviral expression of KDM3A

    Depositor

    Luke Gaughan

    Article

    Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

    Insert

    KDM3A (KDM3A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    two silent mutations to make it siRNA resistant

    Promoter

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

    Plasmid

    #120301

    Purpose

    AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMGIB-3xFLAG-dyskerin

    Plasmid

    #53607

    Purpose

    Retroviral expression of human dyskerin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    dyskerin (DKC1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLenti HRE mutant-Luc pGK Hygro

    Plasmid

    #118707

    Purpose

    reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HREmutant-Luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-Tet-On-shRab27a

    Plasmid

    #120930

    Purpose

    conditionally knockdown Rab27a in mouse cell lines

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    shRab27a (Rab27a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hWIP1 FLAG

    Plasmid

    #28105

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. hRad51(S208E-A209D)–Cas9(D10A)

    Plasmid

    #125561

    Purpose

    Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti Dest BlastR R1-R2

    Plasmid

    #84574

    Purpose

    Gateway destination vector expressing Blasticidin resistance under a PGK promoter

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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