We narrowed to 9,859 results for: Coli

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pRS303-LacZ

Plasmid

#1169

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

LACZ

Tags

GAL1:10 promoter

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJOE3075.3

Plasmid

#135084

Purpose

L-rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Wegerer et al BMC Biotechnol. 2008 Jan 14;8:2. doi: 10

Insert

P-rhaP-BAD-malE

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNK141b2

Plasmid

#156375

Purpose

Expresses orthogonal RBS mTagBFP reporter

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

mTagBFP

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRM2

Plasmid

#8486

Depositor

Nancy Craig

Article

McKown et al J Bacteriol 1988 Jan;170(1):352-8.

Insert

attTn7 (tnsA Bacteria)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pNIC-NHSTIIT-AVITAG-GG

Plasmid

#215802

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pC23-LIC-His-FLAG

Plasmid

#165477

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-2u

Plasmid

#14479

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

5AA

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#113252

Purpose

pUC19 backbone with E. coli CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

E. coli CRISPR array

Use

Integration substrate

Promoter

NA

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-170nDelta13V

Plasmid

#18018

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Tags

None

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-GST-TEV-AVITAG-GG

Plasmid

#215809

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDBD-M

Plasmid

#50572

Purpose

Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pCYP03

Plasmid

#212836

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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