We narrowed to 37,723 results for: Spr

1. pJF093

   Plasmid

   #113323

   Purpose

   Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9 and MCP-SoxS_R93A
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   R93A

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD7

   Plasmid

   #73916

   Purpose

   gRNA vector for AsCpf1

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   tRNA:hAsCpf1-gRNA:tRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDB4280

   Plasmid

   #98699

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p(bhsp68-Cas9)

   Plasmid

   #65959

   Purpose

   Tribolium basal heat shock promoter driving Cas9

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2015 Jul 9. pii: dev.125054

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   nuclear localization sequence

   Expression

   Insect

   Promoter

   bhsp

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMVP-Cas9N

   Plasmid

   #80934

   Purpose

   Plasmid that expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMVP-Cas9C

   Plasmid

   #80939

   Purpose

   Plasmid that expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_CEBPB_1

   Plasmid

   #64036

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_CEBPB_2

   Plasmid

   #64047

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-MsgRNA-EGFP

    Plasmid

    #80794

    Purpose

    For insertion of gRNA array containing 11-30 gRNA modules

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. sg_shuttle_RFP657

    Plasmid

    #134968

    Purpose

    Lentiviral vector for sgRNA expression

    Depositor

    Beat Bornhauser

    Article

    Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6-sgRNA; EFS-RFP657

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD227

    Plasmid

    #113318

    Purpose

    AraC-pBAD-dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    R93A

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-L3US2-RFP-HDAC3

    Plasmid

    #83966

    Purpose

    Lentiviral CRISPR HDAC3 dual gRNA targeting vector

    Depositor

    Eleanor Chen

    Insert

    HDAC3 dual gRNAs (HDAC3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH1v1

    Plasmid

    #60244

    Purpose

    gRNA expression by the H1 promoter

    Depositor

    Donald Zack

    Article

    Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 promoter

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMZ283

    Plasmid

    #52224

    Purpose

    Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColE1_sgRNA_pos9_AmpR

    Plasmid

    #119152

    Purpose

    Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 9 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_CREB1_2

    Plasmid

    #64940

    Purpose

    Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR1002

    Plasmid

    #71820

    Purpose

    Express Streptococcus pyogenes Cas9

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPyCas9
    

    Use

    CRISPR

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMB67

    Plasmid

    #47947

    Purpose

    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Promoter

    hsp-16.48

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJF131B

    Plasmid

    #113326

    Purpose

    pTet-W108 scRNA.b2, pTet-RR2-sgRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2 and sgRNA_RR2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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