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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,800 results for: cloning

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Showing: 2341 - 2360 of 63800 results

  3. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Publication
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131464-plasmid-map-sequence-id-261686

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  4. HR180-LGR5-iCT

    Plasmid
    #129094
    Purpose
    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus
    Depositor
    Toshiro Sato
    Publication
    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    Use
    Homologous recombination donor plasmid
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129094-plasmid-map-sequence-id-252095

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  7. Lamin A-mRFP

    Plasmid
    #124268
    Purpose
    Wild-type Lamin A, C-terminally tagged with mRFP
    Depositor
    Eric Schirmer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124268-plasmid-map-sequence-id-241940

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  9. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Publication
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

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  12. pMH-SFB-BRCA2

    Plasmid
    #99395
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2
    Depositor
    Junjie Chen, Michael Huen
    Publication
    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRCA2 (BRCA2 Human)
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99395-plasmid-map-sequence-id-190616

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  13. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92396-plasmid-map-sequence-id-178234

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  14. pCMV-DeAct-SpvB

    Plasmid
    #89446
    Purpose
    Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89446-plasmid-map-sequence-id-177125

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  16. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  17. mKate2-P2A-APEX2-2xFYVE_hrs

    Plasmid
    #67663
    Purpose
    Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Publication
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKate2-P2A-APEX2-2xFYVE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67663-plasmid-map-sequence-id-117188

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Showing: 2341 - 2360 of 63800 results