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pT7-EGFP-C1-HsAGO4_F

Plasmid

#146317

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTW072

Plasmid

#190247

Purpose

T-DNA for arabidopsis transformation

Depositor

Feng Zhang

Article

Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

Insert

gRNA, Cas9, firefly luciferase, bar

Use

CRISPR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTW069

Plasmid

#190246

Purpose

T-DNA for arabidopsis transformation

Depositor

Feng Zhang

Article

Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

Insert

gRNA, Cas9, firefly luciferase, bar

Use

CRISPR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBA2705

Plasmid

#189998

Purpose

Inducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Inserts

FBP75
VhhGFP4

Use

Expression vector for trypanosoma brucei

Mutation

N-terminal 600 bp of FBP75

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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NK88

Plasmid

#176612

Purpose

To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

DNA-CARy- aTM (aa291-404)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-N-term_F

Plasmid

#146313

Purpose

Mammalian Expression of HsTNRC6C-Nterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-Nterm (TNRC6C Human)

Expression

Mammalian

Mutation

N548S

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179029

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179028

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO4_C

Plasmid

#146085

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rhoa

Plasmid

#163605

Purpose

Expresses RhoA Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rhoa (Rhoa Mouse)

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-LifeAct-EGFP

Plasmid

#163608

Purpose

Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

LifeAct

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM62636

Plasmid

#169095

Purpose

Low copy MoClo level P binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Article

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Expression

Yeast

Mutation

WT

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-1

Plasmid

#111306

Purpose

prp8-1

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-1 ( G1801D )

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-Snu114-fSNAPc

Plasmid

#106989

Purpose

open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Snu114

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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