We narrowed to 16,572 results for: nans
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Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPEP-TEV cNup98 (1-498)
Plasmid#38037DepositorInsertNucleoporin 98 (NUP98 Human)
TagsHis-tag, 3CysteineExpressionBacterialMutationamino acids 1-498PromoterT7Available SinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EPG
Plasmid#117978PurposeExpress EPG in mamalian cellsDepositorInsertElectromagnetic perceptive gene
TagsHIS and v5ExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_Capture1_APOBEC_HA_P2A_mRuby
Plasmid#183901PurposeInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsCapture1_APOBEC_HA_P2A_mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA S31N
Plasmid#19718DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralExpressionMammalianMutationS31N mutant for dominant neg function. NOTE: lo…Available SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-15
Plasmid#228971PurposeFor bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-15
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
ExpressionMammalianAvailable SinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
BLADE
Plasmid#134912PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades systemDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyPromoterU6Available SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable SinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
FB027
Plasmid#119712PurposeMultipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMTDepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
UseSynthetic BiologyAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Stat3-C
Plasmid#13373PurposeRetroviral expression of dominant active mouse Stat3DepositorInsertsignal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
UseRetroviralExpressionMammalianMutationChanged Alanine 661 to cysteine and asparagine 66…Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPFO*
Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
TagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…Available SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2
Plasmid#195526PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoterDepositorInsertAce-mNeon2
UseAAVExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
8138-E02
Plasmid#225659PurposeGateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)DepositorInsertSORE6
UseGateway entry cloneAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralExpressionMammalianPromoterEF1-aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MYRIP
Plasmid#89586PurposeExpression plasmid for a positive control prey protein in NanoSPD 2.0 assays.DepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only