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pBA904

Plasmid

#122238

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

Depositor

Jonathan Weissman

Publication

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs1 in stem loop 2 of the constant region

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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EGFPC1-hMyoX

Plasmid

#47608

Depositor

Emanuel Strehler

Publication

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

MyoX (MYO10 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMXs-IP GFP-NBR1

Plasmid

#38283

Depositor

Noboru Mizushima

Publication

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Next to BRCA1 gene 1 protein (NBR1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Publication

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Tags

FLAG

Expression

Mammalian

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

Plasmid

#21850

Depositor

David Ron

Publication

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

ATF4 (Atf4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

ATF4 5' UTR and AUG fused to Luciferase driv…

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET His6 NusA TEV LIC cloning vector (1N)

Plasmid

#29657

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, NusA, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Publication

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Publication

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLJC6-3XHA-TMEM192

Plasmid

#104434

Purpose

Expresses 3XHA-TMEM192 in mammalian cells

Depositor

David Sabatini

Publication

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

Ubiquitin

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Emerin pEGFP-C1 (637)

Plasmid

#61993

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GAL4UAS

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Publication

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pJ241-FLEX

Plasmid

#18925

Purpose

FLEX switch empty backbone

Depositor

Scott Sternson

Publication

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

FLEX switch

Use

Cre/Lox; Subcloning

Mutation

An internal Spe1 site is used for subcloning a ge…

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV6M-PAK1 T423E

Plasmid

#12208

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Activated Pak1: T423E

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Publication

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PKC alpha CAT

Plasmid

#21234

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Mutation

delta 1-325

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Publication

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pARF6(T27N)-CFP

Plasmid

#11386

Depositor

Joel Swanson

Publication

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 27 to Asparagine.

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Publication

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

T790M

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles