284,499 results

1. pCBC-MT1T2

   Plasmid

   #50593

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T1, gRNA scaffold, OsU3t plus, TaU3p, T2
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-GCaMP6m-Xn

   Plasmid

   #118976

   Purpose

   Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

   Depositor

   Xiaodong Liu

   Article

   Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

   Insert

   GCaMP6m-XN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-GCaMP6m-Xn

   Plasmid

   #118976

   Purpose

   Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

   Depositor

   Xiaodong Liu

   Article

   Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

   Insert

   GCaMP6m-XN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIRV-NFAT-eGFP

   Plasmid

   #118031

   Purpose

   Fluorescent reporter for NFAT activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NFAT binding site

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIRV-NFAT-eGFP

   Plasmid

   #118031

   Purpose

   Fluorescent reporter for NFAT activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NFAT binding site

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM162

   Plasmid

   #173221

   Purpose

   Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Inserts

   ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM162

   Plasmid

   #173221

   Purpose

   Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Inserts

   ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

    Pooled Library

    #101927

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-GFP

    Plasmid

    #96930

    Purpose

    Tunable and temporal expression control of GFP

    Depositor

    Xiaojun Lian

    Article

    Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. XLone-GFP

    Plasmid

    #96930

    Purpose

    Tunable and temporal expression control of GFP

    Depositor

    Xiaojun Lian

    Article

    Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_BiSSTe4_Gq_dTomato

    Plasmid

    #213946

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-nEF-Coff/Fon-ChRmine-oScarlet (AAV8)

    Viral Prep

    #137160-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChRmine-oScarlet (#137160). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Flp (inhibited in the presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    oScarlet (Flp-dependent)

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TLR2-YFP

    Plasmid

    #13016

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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