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pGEL083

Plasmid

#137892

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab4 WT

Plasmid

#37690

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pBW102ParsR-Amp32C

Plasmid

#78637

Purpose

pBW100ParsR carrying 30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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peSpdCas9-J23109

Plasmid

#113153

Purpose

Plasmid constitutively expressing eSpdCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpdCas9

Expression

Bacterial

Mutation

D10A & K810A & H840A & K1003A & R…

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab35 WT

Plasmid

#37741

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pMMB206-CT61

Plasmid

#165410

Purpose

Bacteria gene circuit on low copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

CT61 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Adeno-miR-27b

Plasmid

#112539

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-27b (MIR27B Human)

Use

Adenoviral

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab35 DN

Plasmid

#53495

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Tags

YFP

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pBS-AMV-δ1L9'S-nAchR

Plasmid

#20687

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

H467A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
hs-norpA

Plasmid

#26858

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

no receptor potential A (norpA Fly)

Expression

Insect

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab23 DN

Plasmid

#37727

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S51N (dominant negative)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab23 CA

Plasmid

#37728

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q96L (constitutive active)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-P85A

Plasmid

#33062

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P85A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
Hy_pMT dati MCS circfirefly_1677

Plasmid

#110118

Purpose

Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST YFP Rab23 WT

Plasmid

#53484

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab30 WT

Plasmid

#37735

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab30 (Rab30 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9

Plasmid

#243734

Purpose

PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Truncated_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10

Plasmid

#243735

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Cas9-Pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9

Plasmid

#243737

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike base (Pvc8) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc8-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-Ad5_pvc10-Cas9

Plasmid

#243733

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ276

Plasmid

#217490

Purpose

RADARSv3 driving mNeonGreen

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ275

Plasmid

#217491

Purpose

RADARSv3 driving luciferase

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

gaussia luciferase

Expression

Mammalian

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY240

Plasmid

#239811

Purpose

Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

H3
H2B

Use

Fungal expression

Tags

EGFP and mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY245

Plasmid

#239812

Purpose

Express H3-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY203

Plasmid

#239802

Purpose

Express GUS in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

GUS

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY209

Plasmid

#239804

Purpose

Express mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY219

Plasmid

#239806

Purpose

Express H2B-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY220

Plasmid

#239807

Purpose

Express H3-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY224

Plasmid

#239808

Purpose

Express H2B-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY225

Plasmid

#239809

Purpose

Express H3-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LSM102 CMV-TO-CX3CR1-10aa-PCP (FLP-IN)

Plasmid

#233404

Purpose

CX3CR1-V2-PCP with a 10 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-PCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
YX022 (PB)EF1a-LIDARsensor (hygR)

Plasmid

#233431

Purpose

EF1a-driven LIDAR reporter RNA

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3

Plasmid

#233421

Purpose

IVT template for CCR6-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-CCR6-V2-ADAR2dd-hybridUTR3

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ076 (PB) CMV-mCCL20 (PuroR-BFP)

Plasmid

#233433

Purpose

PiggyBac donor plasmid, membrane-bound CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ033 CMV-TO-CCL20-pre-mGRASP

Plasmid

#233417

Purpose

plasmid expressing membrane-bound CCL20 by fusing with pre-mGRASP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN)

Plasmid

#233437

Purpose

SFFV driven AVPR2-ADAR2dd (as in XZ030)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM

Plasmid

#233428

Purpose

LIDAR reporter RNA with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-sensor(tight)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)

Plasmid

#233438

Purpose

SFFV driven CCR6-ADAR2dd (as in XZ032)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3

Plasmid

#233420

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-LIDARsensor-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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