We narrowed to 4,454 results for: TIL

1. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CVS-N2c-jGCaMP8s

   Plasmid

   #172389

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8s
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK331

   Plasmid

   #59572

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(16) 2xK-turn Kozak EGFP attB2 AscI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RT007-GCN4scFV-VB

   Plasmid

   #163387

   Purpose

   2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CapChR1_pmCherry-N1

   Plasmid

   #188031

   Purpose

   Expresses CapChR1-mCherry in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR1-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   S63D, L132C, T159C and N258E mutations in CrChR2

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-P3nmt1

   Plasmid

   #39280

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF Synaptophysin-GCamp6s

   Plasmid

   #188981

   Purpose

   Presynaptic fluorescent Ca2+ indicator

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Synthetic, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOM-CD63-COM

   Plasmid

   #138350

   Purpose

   Expressing COM-CD63-COM fusion protein in mammalian cells

   Depositor

   Baisong Lu

   Article

   Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

   Insert

   COM-CD63-COM fusion protein
   

   Use

   CRISPR and Lentiviral

   Tags

   COM is fused to the N and C termini of human CD63

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GOMO-GFP

    Plasmid

    #60975

    Purpose

    Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

    Depositor

    Olivier Silvie

    Article

    Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

    Inserts

    GFP
    mCherry
    hDHFR-yFCU
    

    Use

    Plasmodium gene targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CVS-N2c-nl.EGFP-SypGFP

    Plasmid

    #172380

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + SypEGFP
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Tsapphire-StagRFP-SrcAR

    Plasmid

    #173018

    Purpose

    Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Tsapphire-StagRFP-SrcAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTG-mTDG

    Plasmid

    #52266

    Purpose

    Bacterial expression of mouse TDG with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HB501: pMVP (L1-L4) RIP

    Plasmid

    #121688

    Purpose

    pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Rat Ins1 promoter (RIP; β-cell specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCLV3Cas-LT1

    Plasmid

    #210549

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    2bp insertion after AAVS1 target sequence that di…

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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