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pLKO.1 RagD shRNA #1

Plasmid

#30321

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
ZapLck biosensor

Plasmid

#131584

Purpose

Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

Insert

ZapLck biosensor

Use

Lentiviral

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30 PTBP1 isoform1

Plasmid

#108591

Purpose

Recombinant His tagged protein production

Depositor

Chris Smith

Article

Wollerton et al RNA. 2001 Jun;7(6):819-32.

Insert

PTBP1 isoform 1 (PTBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

isoform 1 (isoform c)

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4/hL3MBTL1

Plasmid

#28231

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Irrelevant S49T mutation

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
pCX-Klf5

Plasmid

#66657

Purpose

For expression of mouse Klf5.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf5 (Klf5 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-kanMX6

Plasmid

#26875

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pBMTBX-3

Plasmid

#26074

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 9, 2010

Availability

Academic Institutions and Nonprofits only

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TRPML3-HA

Plasmid

#18832

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
5407 pClpB

Plasmid

#1235

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ClpB

Expression

Bacterial

Mutation

none

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBS mPrx1 (CT#640)

Plasmid

#13961

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 promoter (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 RARalpha

Plasmid

#35557

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pIB3

Plasmid

#25452

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pUASTP2.1

Plasmid

#13842

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

mini-white (w Fly)

Expression

Insect

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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mCD4 e/p Sal-(hCD2)

Plasmid

#24070

Depositor

Dan Littman

Article

Sawada et al Cell. 1994 Jun 17. 77(6):917-29.

Insert

CD2 (CD2 Human)

Tags

mCD 4 promoter

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pLL3.7-EF-EYFP-YAP1_S94A-PolyA

Plasmid

#112286

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Serine 94 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMK1200

Plasmid

#84219

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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YCPlac33-sGFP-VRG4

Plasmid

#25447

Depositor

Benjamin Glick

Article

Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Insert

Synthetic Green Fluorescent Protein - VRG4

Tags

GFP

Expression

Yeast

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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