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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry-NPM1

Plasmid

#194546

Purpose

Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

NPM1 (Npm1 Mouse)

Tags

mCherry

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-HRP-KDEL

Plasmid

#85582

Purpose

Expresses HRP-KDEL in mammalian cells

Depositor

Alice Ting

Insert

HRP-KDEL

Tags

V5

Expression

Mammalian

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Akaluc_SV40:PuroR

Plasmid

#183048

Purpose

Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

AkaLuc

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP (AAV9)

Viral Prep

#107708-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSynapsin- soCoChR-GFP (#107708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin- soCoChR-GFP plasmid DNA. Synapsin-driven somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Promoter

Syn

Tags

GFP

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only
pEXP5-NT/pLux eGFP

Plasmid

#193624

Purpose

Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

eGFP

Tags

6xHis

Expression

Bacterial

Promoter

pLux

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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USP2

Plasmid

#36894

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP2 (USP2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits