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Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 6FLAG
Plasmid#182509PurposeC-SWAT compatible vector for C-terminal tagging with 6 FLAGDepositorInsert6 FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_hRNMT_RAM
Plasmid#213528PurposeExpresses the human RNMT-RAM complexDepositorInsertsUseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCMV UL97 KD
Plasmid#26688DepositorInsertUL97 (UL97 Human Cytomegalovirus (HHV-5))
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant Lys 355 mutated to MetPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A(KaganF58)
Plasmid#52001PurposeExpresses the human MAVS CDS containing the point mutation M1ADepositorInsertMAVS
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2B
Plasmid#105501PurposeExpression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression levelDepositorInsertdH2B
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HHV6 U69 KD
Plasmid#26690DepositorInsertU69 (U69 Human Herpes Virus 6)
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant: Lys 218 mutated to MetPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT SMASh
Plasmid#182523PurposeC-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182524PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 3MYC SMASh
Plasmid#182525PurposeC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).DepositorInsertSMASh Tag
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP DAmP
Plasmid#182511PurposeC-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPR pBAM Ura3
Plasmid#45355DepositorInsertDHFR Ub Mek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MAVS-M142A(KaganF61)
Plasmid#51999PurposeExpresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"DepositorInsertMAVS
UseTagsHAExpressionMammalianMutationMet 142 to AlaPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
UseTagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK084
Plasmid#171356PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Met] A.niger Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK023
Plasmid#171295PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPtRNA[Met] P. rubens Pol-III promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0034-MV_RN
Plasmid#127017PurposeBasic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0034m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A-invitro(KaganE12)
Plasmid#52016PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M1ADepositorInsertMAVS-M1A
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-G67M-invitro(KaganD100)
Plasmid#52014PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation G67MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Gly 67 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-L62M-invitro(KaganD98)
Plasmid#52013PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation L62MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Leu 62 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-E80M-invitro(KaganD99)
Plasmid#52015PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation E80MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Glu 80 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A-invitro(E13)
Plasmid#52017PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M142ADepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0032-MV_RN
Plasmid#127015PurposeBasic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0032m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17
Plasmid#193319PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTagsIAA17ExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT Compatible pAIDA2(6FLAG)
Plasmid#193321PurposeC-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.DepositorInsertTAP-AID*-6FLAG
UseTagsTAP-AID*-6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6MYCExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6FLAG osTIR1
Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182516PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3HA
Plasmid#182517PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3HAExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3MYC
Plasmid#182518PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182522PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0033-MV_RN
Plasmid#127016PurposeBasic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0033m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS-M1A(KaganE18)
Plasmid#52009PurposeExpresses the human MAVS CDS containing the point mutation M1ADepositorInsertMAVS-M1A
UseRetroviralTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS-M142A(KaganE19)
Plasmid#52010PurposeExpresses the human MAVS CDS containing the point mutation M142ADepositorInsertMAVS-M142A
UseRetroviralTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A(KaganF59)
Plasmid#52062PurposeExpresses the human MAVS CDS containing the point mutation M142A "weak translational context"DepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only