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Showing: 221 - 240 of 1323 results

  1. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  10. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  11. pINDUCER20-hMYOD1-3xHA (#730)

    Plasmid
    #78328
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78328-plasmid-map-sequence-id-424966

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  12. qTAG-N-Blast-3xHA-LMNB1

    Plasmid
    #207778
    Purpose
    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207778-plasmid-map-sequence-id-416192

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  14. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227907
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227907-plasmid-map-sequence-id-452503

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  15. pCS2+N-3xHA Cloning Vector

    Plasmid
    #196653
    Purpose
    N-terminal 3xHA mammalian expression plasmid
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196653-plasmid-map-sequence-id-387128

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  20. pINDUCER20-hMYOG-3xHA (#733)

    Plasmid
    #78342
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYOG ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOG (MYOG Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 1323 results