We narrowed to 2,123 results for: D10

1. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Promoter

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

   Plasmid

   #208290

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpCas9(D10…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L

   Plasmid

   #12474

   Depositor

   David Baker

   Insert

   Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   b19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K…

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210735

   Purpose

   Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Gollum sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210736

   Purpose

   Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Merry sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PROM_10

   Plasmid

   #201301

   Purpose

   D10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM part

   Depositor

   Benjamin Schwessinger

   Insert

   D10: Promoter of TraesCS3D02G517600
   

   Use

   Tags

   Expression

   Plant

   Mutation

   removal of BsaI and BpiI sites

   Promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2P65

   Plasmid

   #60113

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pv089580:MAC01Q-D10:C26843
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pMT-TAK1-V5-His

   Plasmid

   #63753

   Purpose

   Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   TGF-β activated kinase 1 (Tak1 Fly)
   

   Use

   Tags

   V5-His

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti dCAS-VP64_Blast (Lentiviral Prep)

    Viral Prep

    #61425-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Promoter

    EF1a

    Tags

    None

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

    Viral Prep

    #61422-LVC

    Purpose

    Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Promoter

    EF1a

    Tags

    None

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Opto-GPR139

    Plasmid

    #106044

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR139
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSJ392

    Plasmid

    #159722

    Purpose

    Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    GID10 (GID10 Budding Yeast)
    

    Use

    Yeast two-hybrid assay

    Tags

    3xflag and Gal4AD

    Expression

    Yeast

    Mutation

    remove stop codon

    Promoter

    ADH1

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOSGE_CUS1D07

    Plasmid

    #71085

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED10 (MED10 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ265N2

    Plasmid

    #174001

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ265O2

    Plasmid

    #174002

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ265L2

    Plasmid

    #174000

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH-ABE8e

    Plasmid

    #200774

    Purpose

    For plant adenine base editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

    Insert

    nCas9(D10A)-TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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