We narrowed to 2,021 results for: D10

Showing: 221 - 240 of 2021 results

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TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa

Plasmid

#182897

Purpose

Mammalian expression of TH mouse mAb LC gene

Depositor

Heng Zhu

Insert

anti-TH (human) light chain (TH Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

No

Promoter

CMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAD108_Arg273His_pCMV6Entry-hIRG1

Plasmid

#124878

Purpose

Expresses hCAD Arg273His mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Arg273 to His

Promoter

CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_5

Plasmid

#91170

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Hoxd10

Plasmid

#45721

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

Hoxd10 (HOXD10 Chicken)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

MED10 (Med10 Mouse)

Tags

2xHA

Expression

Insect

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-OGT1

Plasmid

#29760

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

OGT1 (OGT Human)

Tags

Flag

Expression

Mammalian

Mutation

N764D and K1010E

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Mutation

D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Tags

His6-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk1 WT HA

Plasmid

#27652

Depositor

Greg Enders

Insert

Cdk1 (CDK1 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA Flag Lamp2b-HA

Plasmid

#71292

Purpose

Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

Flag, HA, and Lamp2 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d10-52-3xHA (Δα1)

Plasmid

#232004

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the first alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ10-52

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

Plasmid

#165444

Purpose

Expresses ACX, rXRCC1 in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rXRCC1 (Apobec1 Streptococcus pyogenes, Rat, Synthetic)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 beta4

Plasmid

#16037

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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