We narrowed to 12,240 results for: PAC;

1. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. H9 F42A pAcGP67a

   Plasmid

   #41810

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   H9 Super-2 (IL2 Human)
   

   Use

   Tags

   8x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pX330-HypaCas9

   Plasmid

   #108302

   Purpose

   pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC059-HisMS2_PLP_NucPhos_pac

   Plasmid

   #155039

   Purpose

   Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

   Depositor

   Paul Freemont

   Article

   Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

   Inserts

   Maturation Protein
   Coat Protein Dimer
   Nucleocapsid Phosphoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Remove TypeIIs Restriction Sites

   Promoter

   T7

   Available since

   Sept. 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAcGP67A-TfR

   Plasmid

   #12130

   Depositor

   Pamela Bjorkman

   Article

   Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

   Insert

   transferrin receptor (TFRC Human)
   

   Use

   Tags

   6X-His tag, Factor Xa cleavage site, and leader p…

   Expression

   Insect

   Mutation

   The portion of the human TfR gene encoding residu…

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-PACT

   Plasmid

   #50352

   Purpose

   bacterial expression of PACT

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   PACT (PRKRA Human)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc V5 dDispatched WT HA

   Plasmid

   #126405

   Purpose

   Expression of V5 dDispatched WT HA in Drosophila cells lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   d-Dispatched1 (disp Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPACE (pRZ1813)

   Plasmid

   #140242

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pActP-dtrpA1

    Plasmid

    #64713

    Purpose

    inducing calcium influx in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    dtrpA1 (TrpA1 )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pActin-miRFP670nano

    Plasmid

    #127428

    Purpose

    Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-human beta-actin
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-CoRESTfl

    Plasmid

    #109156

    Purpose

    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGFP-C2-hSphK2

    Plasmid

    #84370

    Purpose

    Expression of AcGFP-SphK2 fusion protein in mammalian cells

    Depositor

    Anthony Don, Hugh Rosen

    Article

    Don et al Biochem Biophys Res Commun. 2009 Feb 27;

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pFGC5941-PacI

    Plasmid

    #44182

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYC-GST

    Plasmid

    #62329

    Purpose

    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Glutathione S-transferase (GST) tag and TEV prote…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac0

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcBac1-haloTyrRS C6

    Plasmid

    #141174

    Purpose

    Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyr tRNA synthetase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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