We narrowed to 1,046 results for: Psp;

1. pSPL3-hRPH3A_c.1853Gmut

   Plasmid

   #190479

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Expression

   Mammalian

   Mutation

   c.1853A>G

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP64-Lifeact-GFP

   Plasmid

   #135601

   Purpose

   Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   Lifeact-GFP
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   EGFP

   Promoter

   Unknown

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-puro-a5 guide B

   Plasmid

   #194011

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide B)
   

   Use

   CRISPR

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-puro-aV guide A

   Plasmid

   #194008

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide A)
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-puro-aV guide B

   Plasmid

   #194009

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide B)
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9n(BB)-2A-puro-a5 guide A

   Plasmid

   #194010

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide A)
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCT-GAL93-PSPC1

   Plasmid

   #46321

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   PSPC1 (PSPC1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pSpot-Tag-Actin

   Plasmid

   #117060

   Purpose

   For mammalian expression of beta-actin with N-terminal Spot-Tag

   Depositor

   Felix Hartlepp

   Insert

   actin beta (ACTB Human)
   

   Tags

   Spot-Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64-Vasa-GFP

    Plasmid

    #135595

    Purpose

    Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Yajima et al Development. 2015 Jun 1;142(11):1960-70.

    Insert

    Vasa-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    EGFP

    Promoter

    SP6

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpot-Tag-Lamin

    Plasmid

    #117062

    Purpose

    For mammalian expression of lamin B1 with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    Lamin B1 (LMNB1 Human)
    

    Tags

    Spot-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64T tBMPR (DM#135)

    Plasmid

    #15067

    Depositor

    Douglas Melton

    Article

    Graff et al Cell. 1994 Oct 7. 79(1):169-79.

    Insert

    BMP receptor (bmpr1a Frog)
    

    Expression

    Bacterial

    Mutation

    Truncated dominant negative form

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP64-membrane-mCherry

    Plasmid

    #135598

    Purpose

    Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    membrane-mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    mCherry

    Promoter

    Unknown

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP64-AGS-mCherry

    Plasmid

    #135596

    Purpose

    Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    AGS-mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    mCherry

    Promoter

    Unknown

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64T-ythdf2-3xflag

    Plasmid

    #164489

    Purpose

    Expresses zebrafish ythdf2 with 3x flag tag, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    ythdf2 (ythdf2 Zebrafish)
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    3x-flagtag

    Promoter

    SP6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCAS9n(BB)-2A-puro DNM1, A

    Plasmid

    #107795

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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