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Showing: 221 - 240 of 475 results
  1. TFORF1316

    Plasmid
    #143787
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    RB1CC1 (RB1CC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pEUI(+)

    Plasmid
    #111834
    Purpose
    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SV40, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pENTR-EUI

    Plasmid
    #111835
    Purpose
    Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Entry vector for expression
    Tags
    Expression
    Mutation
    Promoter
    CMV, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pRS415-KEX2

    Plasmid
    #10996
    Depositor
    Insert
    KEX2 (KEX2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
  7. R777-E305 Hs.SCRIB

    Plasmid
    #70589
    Purpose
    Gateway ORF clone of human SCRIB [NM_182706.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    SCRIB (SCRIB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
  9. R777-E171 Hs.PIK3R1

    Plasmid
    #70455
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. R777-E306 Hs.SCRIB-nostop

    Plasmid
    #70590
    Purpose
    Gateway ORF clone of human SCRIB [NM_182706.4] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    SCRIB (SCRIB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. R777-E172 Hs.PIK3R1-nostop

    Plasmid
    #70456
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pLenti4/V5-DEST-P85(G376R)

    Plasmid
    #40220
    Depositor
    Insert
    P85(G376R) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Glycine 376 to Arginine
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 475 results