We narrowed to 9,830 results for: pET

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pETM33_Nsp5_Mpro

Plasmid

#156475

Purpose

Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28CLY1

Plasmid

#21760

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible one SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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CyPET-Arf6

Plasmid

#18840

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

CyPET-Arf6 (ARF6 Human)

Expression

Mammalian

Mutation

The sequence for the fluorescent protein CyPET wa…

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only
pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_1

Plasmid

#78198

Purpose

Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A197C)

Expression

Bacterial

Mutation

A197C (please see depositors comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pPET-PKR/PPase

Plasmid

#42934

Depositor

James Cole

Article

Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

Inserts

protein kinase R (EIF2AK2 Human)
phage lambda phosphatase

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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pTSA-13

Plasmid

#17327

Depositor

Takeshi Sano

Article

Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pMJ922

Plasmid

#78312

Purpose

Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

Depositor

Martin Jinek

Article

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-EGFP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET28HIS-hAPE1

Plasmid

#70757

Purpose

bacterial expression of human APE1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

APEX1 (APEX1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-KSRP

Plasmid

#23019

Depositor

Douglas Black

Article

Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

Insert

KSRP (KHSRP Human)

Tags

His

Expression

Bacterial

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pYpet-PBD

Plasmid

#22781

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET42b-ABE7.10

Plasmid

#120398

Purpose

Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

Junjiu Huang

Article

Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

Insert

ABE7.10

Tags

His

Expression

Bacterial

Mutation

Mammalian codon-optimized

Promoter

T7

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(WT)

Plasmid

#22782

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (wt) (RAC1 Human)

Tags

CyPet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only