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We narrowed to 2,492 results for: PUC19

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Showing: 2381 - 2400 of 2492 results

  1. qTAG-C-Solo-miRFP670nano3-H2BC11

    Plasmid
    #227331
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227331-plasmid-map-sequence-id-452128

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  2. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  3. pToxamEXP4CUP1

    Plasmid
    #218284
    Purpose
    Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218284-plasmid-map-sequence-id-433686

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  4. qTAG-N-Blast-V5-LMNB1

    Plasmid
    #207779
    Purpose
    Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207779-plasmid-map-sequence-id-416191

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  5. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid
    #207766
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207766-plasmid-map-sequence-id-413792

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  6. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185869-plasmid-map-sequence-id-370814

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  7. pIALD2HMGr

    Plasmid
    #185867
    Purpose
    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185867-plasmid-map-sequence-id-370811

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  11. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  15. pTP63-donor

    Plasmid
    #132477
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132477-plasmid-map-sequence-id-273771

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  16. pZNF215-donor

    Plasmid
    #113804
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP
    Depositor
    Kevin White
    Insert
    ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
    Use
    CRISPR
    Mutation
    rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113804-plasmid-map-sequence-id-231033

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  17. pZNF211-donor

    Plasmid
    #112382
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF211 with GFP
    Depositor
    Kevin White
    Insert
    ZNF211 homology arms flanking EGFP-IRES-Neo cassette (ZNF211 Human)
    Use
    CRISPR
    Mutation
    hg38:19:57641976:T>C, hg38:19:57641979:A>T,…
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112382-plasmid-map-sequence-id-229835

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  18. pZNF766-donor

    Plasmid
    #112352
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF766 with GFP
    Depositor
    Kevin White
    Insert
    ZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
    Use
    CRISPR
    Mutation
    hg38:19:52290357:G>T, hg38:19:52290373:A>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112352-plasmid-map-sequence-id-229667

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  19. pZNF84-donor

    Plasmid
    #112330
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP
    Depositor
    Kevin White
    Insert
    ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
    Use
    CRISPR
    Mutation
    rs623100, hg38:12:133058872:T>C, hg38:12:13305…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112330-plasmid-map-sequence-id-228558

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Showing: 2381 - 2400 of 2492 results