We narrowed to 22,151 results for: tes

1. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

   Plasmid

   #217723

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-523

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJG80

   Plasmid

   #89282

   Purpose

   Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS NL4-3 envFS eGFP Δnef/U3

   Plasmid

   #101346

   Purpose

   Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta nef/LTR

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrcHisA-rat-PTP1B

   Plasmid

   #35987

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

   Insert

   protein tyrosine phosphatase 1B (Ptpn1 Rat)
   

   Use

   Tags

   His and Xpress Epitope Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB6HindIII-rat FcRn

   Plasmid

   #121671

   Purpose

   Expresses rat neonatal Fc receptor in mammalian cells

   Depositor

   Pamela Bjorkman

   Article

   Tesar et al Traffic. 2006 Sep;7(9):1127-42.

   Insert

   rat neonatal Fc receptor (Fcgrt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD6-pGRR-r11-r10-yeGFP

   Plasmid

   #74368

   Purpose

   pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   yeGFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGRR-r11-r10

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

   Plasmid

   #217689

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE1 (EMR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-584

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV 4xSTAR-mTFP-NLS-blast

   Plasmid

   #136257

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTFP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. hME-pOX

    Plasmid

    #178181

    Purpose

    Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

    Depositor

    Ming-Feng Tsai

    Article

    Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

    Insert

    human MCU-EMRE fusion protein
    

    Use

    Tags

    1D4

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrc99-Rat FABP2

    Plasmid

    #13578

    Depositor

    Martin Scanlon

    Article

    Velkov et al J Biol Chem. 2005 May 6. 280(18):17769-7

    Insert

    intestinal fatty acid binding protein (Fabp2 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pHD-mW-attPCC-FRT (pTL717)

    Plasmid

    #115158

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    mW attP-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCALPS_miR-146aSponge_mCherry_GFP

    Plasmid

    #149718

    Purpose

    Lentiviral vector expressing a miR-146a decoy sponge

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    14X miR-146a binding sites
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G to T mutation in position 75 of the insert

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMOD8A-iRGR-r11

    Plasmid

    #74369

    Purpose

    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r11
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AHD1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Use

    Tags

    yEGFP reporter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pITGFP3

    Plasmid

    #65452

    Purpose

    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Use

    Tags

    yEGFP-CLN2PEST reporter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pJL6

    Plasmid

    #184134

    Purpose

    For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    CELE_VW02B12L.1 (vha-6 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Pvha-6 (894bp)

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2 8xSTAR-mScarletI. PGK-puro

    Plasmid

    #136266

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV Cyclin D2-long-mut

    Plasmid

    #21657

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-15/16 and let-7 binding sites

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge