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We narrowed to 19,025 results for: otos

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Showing: 3601 - 3630 of 19025 results

  2. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  3. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  10. pJS288

    Plasmid
    #200714
    Purpose
    To express yeast Hxt1 hexose transporter in yeast
    Depositor
    Jens Boch
    Article
    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi
    Inserts
    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    domesticated for MoClo cloning (removal of intern…
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    200714-plasmid-map-sequence-id-404927

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  13. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  23. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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Showing: 3601 - 3630 of 19025 results